THE 2-MINUTE RULE FOR 2-FDCK HCL

The lab says fixation will trigger the dyes to leak from the cells. I feel the assumption is the fact fixation could damage mobile membranes. I did locate a paper which describes fixation using methanol or acetic acid, just after staining by using a DCFDA spinoff. DCFDA is the reagent that stains ROS during the kit ab113851, and I think that it is